Preparation of Amperometric Biosensor Based on Organic-inorganic Hybrid System

Integration of organic and inorganic material is a promising method for obtaining unusual functional material. It has been investigated that a hybrid system of oxidative enzyme and Prussian blue (PB, FeⅢ4[FeⅡ(CN)6]3) provides an amperometric biosensor working at a very low potential range (0.0 V vs. Ag/AgCl) due to the catalytic function of PB. Recently, we developed a new fabrication method through a combination of Langmuir-Blodgett (LB) technique and self-assembled monolayers (SAMs) technique. In this method, an enzyme of glucose oxidase was chemically bonded with -COOH terminal group of SAM, after that, PB nano-clusters were electrostatically immobilized in the octadecyltrimethylammonium LB film. For the glucose biosensor prepared with this method, we observed a clear response current generation depending on the glucose concentration at a low potential range, a stable linear relationship between the glucose concentration and output current, and a high stability of the sensor performance.