RSM Optimized Media to Increase the Antibacterial Activity of Wild and Mutated Strain of Nocardiopsis VITSRTB

The aim of the present study was to increase the production of antibacterial compound from marine actinobacteria by the effect of chemical mutation as well as optimization of media using Response Surface Methodology (RSM). The efficiency of the antibacterial compound was tested against clinical isolate of Pseudomonas aeruginosa. Mutational effect was analysed by Random Amplified Polymorphic DNA (RAPD). Solvent extraction of the compound was done by using ethyl acetate and further analysed by Gas Chromatography-Mass Spectrophotometry (GC-MS). In primary screening among ten isolates SRTB3 showed maximum antibacterial activity (12 ± 0.40 mm) and the strain was further phenotypically characterized and identified as Nocardiopsis VITSRTB (HF546396). Antibacterial activity was found to be increased after optimization in both wild (22.3 ± 3.07 mm) and chemically mutated (30 ± 3.67 mm) strain of Nocardiopsis VITSRTB (HF546396). The bioactive compounds which were obtained by partial purification are also reported to have antibacterial activity. Thus, this study concludes that marine Nocardiopsis VITSRTB (HF546396) is having good antibacterial activity against P. aeruginosa which can be significantly enhanced by combinational effect of mutation and media optimization by using RSM.