Soybean Allergy: IgE Epitopes of Glycinin (gly M 6), an Important Soybean Allergen

Methods 11 subjects with clinically confirmed soybean allergy and sIgE against rGly m 6 were enrolled in this study. Synthetic overlapping peptides (15-mers, 4 aa offset) representing the amino acid sequences of G1 and G5, two representative subunits of Gly m 6, were spotted onto CelluSpotTM slides and analyzed for IgE-binding, respectively. The sequences of IgE-binding peptides were allocated to the respective surface areas of the 3D-structures of G1 and G5. Allergenspecific IgE-binding to Gly m 6 peptides was verified by inhibition with rG1, rG5, and soy extract, respectively.