Wheat Leaf Lipids During Heat Stress: I. High Day And Night Temperatures Result In Major Lipid Alterations

Understanding how wheat (Triticum aestivum L.) plants under high temperature (HT) regulate lipid composition is critical to developing climate-resilient varieties. We measured 165 glycerolipids and sterol derivatives under optimum and high day and night temperatures in wheat leaves using electrospray ionization-tandem mass spectrometry. Levels of polar lipid fatty acyl chain unsaturation were lower in both heat-tolerant genotype Ventnor and susceptible genotype Karl 92 under HT, compared with optimum temperature. The lower unsaturation was predominantly because of lower levels of 18:3 acyl chains and higher levels of 18:1 and 16:0 acyl chains. Levels of 18:3-containing triacylglycerols increased threefold/more under HT, consistent with their possible role in sequestering fatty acids during membrane lipid remodelling. Phospholipids containing odd-numbered or oxidized acyl chains accumulated in leaves under HT. Sterol glycosides (SG) and 16:0-acylated sterol glycosides (ASG) were higher under HT than optimum temperatures. Ventnor had lower amounts of phospholipids with oxidized acyl chains under HT and higher amounts of SG and 16:0-ASG than Karl 92. Taken together, the data demonstrate that wheat leaf lipid composition is altered by HT, in which some lipids are particularly responsive to HT, and that two wheat genotypes, chosen for their differing physiological responses to HT, differ in lipid profile under HT.Understanding how wheat plants under high temperature regulate lipid composition is critical to developing climate-resilient varieties. Lipid remodeling under high-temperature stress in heat-tolerant and susceptible genotypes resulted in decreases in the amounts of more unsaturated lipid species and increases in the amounts of less unsaturated lipid species, sterol glycosides, 16:0-acylated sterol glycosides, 18:3-acyl-containing triacylglycerols, and phospholipids containing odd-numbered or oxidized acyl chains. The two analyzed wheat genotypes, chosen for their differing physiological responses to high temperature, differed in lipid profiles under high temperature.