Simple Micropropagation by Indirect Somatic Embryogenesis in Sugarcane (Saccharum officinarum L.)

The simple method for producing sugarcane somatic embryos was developed by reducing concentration of 2,4-D. Callus of sugarcane was maintained on MS medium supplemented by 3 ppm of 2,4-D (D3). The callus surface changed into white opaque colour (white nodule) when the concentration of 2,4-D was decreased to 1.5 ppm (D1.5). Thereafter, the somatic embryos developed from the white nodule. There are various developmental stage of sugarcane somatic embryos on D1.5 medium, namely globular, early scutellar, late scutellar, coleoptilar embryos. When the 2,4-D was omitted from the medium (D0), the coleoptilar somatic embryos germinated , formed shoot and root. The germinated somatic embryos were growing well forming plantlet. This simple method offers a convenient tool for micropropagation of sugarcane.