Essential Oil From Flowers Of Solanum Stipulaceum: Composition, Effects Of Gamma-Radiation, And Antileukemic Activity

The composition of essential oils from flowers of Solanum stipulaceum Roem & Schult collected in May and September was studied for the first time. Effects of gamma-radiation on volatile constituents were investigated by gas chromatography (GC) with flame ionization detector (FID). In addition, the antileukemic activity was studied by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay against human cell lines HL-60 and THP-1. The main constituents of the essential oil of flowers collected in May were beta-caryophyllene (25.8%), gamma-gurjunene (11.9%), and beta-gurjunene (8.2%), whereas the essential oil of flowers collected in September was mainly composed of beta-caryophyllene (26.5%), caryophyllene oxide (11.0%), and gamma-gurjunene (10.0%). The main components of essential oil from flowers collected in September were not significantly affected by gamma-radiation at lower doses than 10.0 kGy. No cytotoxic activity in leukemic cell lines was observed for non-irradiated samples. However, irradiated samples exhibited slightly cytotoxic activity.