The application of high resolution diffusion NMR for the characterisation and quantification of small molecules in saliva/dentifrice slurries

The application of DOSY (Diffusion Ordered Spectroscopy) NMR as a technique for the virtual separation of toothpaste adjuvants in model saliva is reported for the first time. In addition, the scope and limitations of DOSY NMR are considered using the DOSY Tool Box processing software, as is the quantification of the adjuvants and components of saliva by quantitative NMR (qNMR). These techniques represent a new and powerful tool for the evaluation of complex mixtures of natural products with a view to identifying biomarkers for disease within the oral cavity.