Insertion Sequence Based Molecular Characterization of Burkholderia mallei NCTC 3709 Strain

Present study evaluated nucleotide sequence variability in 3′ end of insertion sequence IS 407 A in B. mallei NCTC 3709 strain and compared with other B. mallei strains reported worldwide. This insertion sequence IS 407 A was found conserved among all B. mallei strains studied while it was found absent in B. pseudomallei . Flagellar gene (motility gene) showed single nucleotide polymorphism in B. mallei strains. This insertion sequence can be used as a molecular signature for B. mallei organism and it can be successfully used in development of multiplex PCR for simultaneous detection of multiple equine pathogens like B. mallei (glanders), B. pseudomallei (Melioidosis) and Streptococcus equi (strangles).