Sa1173 Early Maternal Separation Induced Commensal E. Coli Overgrowth Associated with Specific Immune Response in Mice at Adulthood

resolution examination.Areas of concern were photo-documented.2.5cc of 10% fluorescein was infused intravenously and both normal appearing tissue and the area of concern were imaged and recorded using the pCLE probe.The area of concern was then biopsied and the biopsies were read by a single pathologist.The pCLE images were examined by a single physician familiar with pCLE technology.Results: 12 patients were enrolled.Average age was 50 years.Five of the 12 patients had lesions with features of high grade dysplasia on biopsy (Anal Intraepithelial Neoplasia 2 or 3 (AIN2/3)).Normal squamous tissue was wellimaged in all patients in non-suspicious areas of "control".Confocal findings in areas of concern included the following: 1) pleomorphic cells with loss of regular squamous architecture (5/5 patients -100%) 2) dark spots, potentially representing gland formation (3/5 patients -60%) 3) large or torturous blood vessels (4/5 patients -80%).4) Dark bands (1/ 5 patients -20%).Quality of imaging was deemed adequate and interpretable in all patients with HGAIN lesions.Conclusions: Distinctive features on pCLE can be found in lesions suspicious for HGAIN in a population of HIV-infected males.If sensitive and specific, pCLE may obviate the need for anal biopsy allowing real-time treatment.