MP34-18 IN VITRO STUDY, THE ROLE OF RHO-KINASE IN URETERAL SMOOTH MUSCLE RELAXATION WITH TAMSULOSIN AND TERPENE MIXURE (ROWATINEX¢Ç)

You have accessJournal of UrologyStone Disease: Basic Research II1 Apr 2015MP34-18 IN VITRO STUDY, THE ROLE OF RHO-KINASE IN URETERAL SMOOTH MUSCLE RELAXATION WITH TAMSULOSIN AND TERPENE MIXURE (ROWATINEX¢Ç) Jea Whan Lee, Tae Hoon Oh, Whi-An Kwon, Seung Chol Park, Hee Jong Jeong, and Ill Young Seo Jea Whan LeeJea Whan Lee More articles by this author , Tae Hoon OhTae Hoon Oh More articles by this author , Whi-An KwonWhi-An Kwon More articles by this author , Seung Chol ParkSeung Chol Park More articles by this author , Hee Jong JeongHee Jong Jeong More articles by this author , and Ill Young SeoIll Young Seo More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1314AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Rho-kinase(ROK) regulate the phosphorylation of myosin light chain through either phosphorylation and subsequent inhibition of myosion phosphatase or direct phosphorylation of Myosin Light Chain on Ser 19 and Thr 18. It has been shown that ROK is important in the regulation of urinary bladder SM contraction and tone. In this study, we focused on the expression of ROK isoforms in response to Tamsulosin and Terpene mixure (Rowatinex¢ç). METHODS We obtained ureteral segments by incising the ureter from renal pelvis to bladder from freshly killed eight-week-old rabbit. The ureter divided into two segments; upper and low. We prepared total protein extracts from the each ureter smooth muscle tissues. The drugs were added to aerated Krebs buffer (95% oxygen and 5% carbon dioxide) at a constant temperature of 37° in increasing concentrations and applied consecutively with a 10-min washout with Kerbs buffer between eack concentration. Only one drug was applied to each ureteric sample, to prevent any risk of cross-reactivity between drugs. We used Western blotting to determine the relative levels of ROK isoform expression at the each drug density. Statistical analysis was performed using the unpaired Student's t test; p<0.05 was considered statistically different. RESULTS In Tamsulosin group, upper ureter at high dose was significantly decreased ROKa expression. However in terpene mixture (Rowatinex¢ç) group, upper ureter at middle and high dose was significantly decreased ROKa expression and lower ureter at middle and high dose was significantly increased ROKa expression (p<0.05). CONCLUSIONS Tamsulosin in upper ureter at high dose, and terpene mixture (Rowatinex¢ç) in upper at high dose was decreased ROKa expression. It showed the effect on spontaneous ureteral relaxation. In particular, terpene mixture (Rowatinex¢ç) might have the most ameliorating effect on ureteral smooth muscle relaxation. Future studies are needed to further assess the effects and mechanism of alpha-receptor antagonists on ureteral peristalsis and clinical ureteral stone passage. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e417 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jea Whan Lee More articles by this author Tae Hoon Oh More articles by this author Whi-An Kwon More articles by this author Seung Chol Park More articles by this author Hee Jong Jeong More articles by this author Ill Young Seo More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...