A purified truncated form of yeast Gal4 expressed in Escherichia coli and used to functionalize poly(lactic acid) nanoparticle surface is transcriptionally active in cellulo

Sophie Legaz,Jeanyves Exposito,Agnes Borel, Mariepierre Candusso,Simon Megy,Roland Montserret, Vincent Lahaye,Christophe Terzian,Bernard Verrier

Protein Expression and Purification（2015）

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摘要

•Expression of a yeast minimal Gal4 form in E. coli.•Purification and refolding of an active Gal4 recombinant protein.•Functionalization of Gal4-PLA nanoparticle.•UAS-GFP transcriptional activation with Gal4-PLA nanoparticles.

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关键词

UAS–Gal4 system,Gal4,Escherichia coli,Inclusion bodies,PLA nanoparticle

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