Application of Bacterium HY9 Strain for Diesel Oil Denitrogenation

HY9 was used in the denitrogenation of diesel oil. The nitrogen removal ratio of diesel oil increased from 12.9% to 16.7% when the surfactant Tween80 was added and the optimal addition content of Tween80 was 0.175 g/25 mL diesel oil. Higher shaking speed could reduce the size of emulsion droplets formed by oil and water, and improve the dissolved oxygen content and the nitrogen removal efficiency. The optimal conditions were 3 mL bacterial suspension/100 mL mixed liquor and 250 rpm shaking speed. Repeated denitrification almost has no effect on denitrogenation efficiency.