Towards a vitrification-based cryopreservation protocol for the coral Pocillopora damicornis L.: Tolerance of tissue balls to 4.5M cryoprotectant solutions

Cryobiology(2015)

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摘要
•Tissue balls of Pocillopora damicornis were used to test cryoprotectant toxicity.•Tissue balls were exposed to cryoprotectant solutions with a molarity up to 5.0M.•Tissue balls tolerated binary cryoprotectant solutions with a 4.0M total molarity.•A 4.5M solution was tolerated when molarity was increased in a stepwise manner.•Ethylene glycol and glycerol were the best tolerated cryoprotectants.
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关键词
Coral,Tissue ball,Pocillopora damicornis,Cryoprotectant solutions
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