Increasing Gene Editing Efficiencies in Eukaryotic Cell Lines by Selection of Appropriate CRISPR-Cas9 Reagents

Genetic engineering of living cells is critical for understanding gene function in normal and diseased states. The CRISPR-Cas9 system is widely utilized because of its ease-of-use compared to other gene editing methods. This system requires a complex of Cas9 protein with tracrRNA and a gene-targeting crRNA to introduce DNA double-strand breaks at a specific location in the genome to disrupt protein translation and knockout gene function. For highest gene editing efficiencies, it is essential to choose the best CRISPR-Cas9 reagents for delivery and expression into the cells of interest.