Evaluation of convenient pretreatment protocols for RNA virus metagenomics in serum and tissue samples.

Toon Rosseel,O Ozhelvaci,G Freimanis,Van Borm S

Journal of Virological Methods（2015）

引用 47|浏览9

暂无评分

摘要

•Viral enrichment approaches for RNA virus discovery in clinical samples are compared.•The preferred approach depends on nucleic acid composition of the sample.•Direct sequencing of cDNA is recommended over an extra random PCR amplification step.•Guidelines for more targeted RNA virus discovery strategies are provided.

查看译文

关键词

RNA virus discovery,Clinical samples,Viral metagenomics,Pretreatment,Next generation sequencing,rRNA depletion

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要