Investigation of low recovery in the free drug assay for antibody drug conjugates by size exclusion-reversed phase two dimensional-liquid chromatography.
Journal of Chromatography B（2016）
摘要
•Using 2D-LC to investigate ADCs.•Residual Reactive Protein Sites impacting method performance.•Capping Restores Spike Recovery.
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关键词
Antibody drug conjugates,2D-LC,Hydrophobic interaction chromatography
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