A real-time fluorometric method for the simultaneous detection of cell death type and rate

Sasker Grootjans,Behrouz Hassannia,Iris Delrue,Vera Goossens,Bartosz Wiernicki,Yves Dondelinger,Mathieu J M Bertrand,Dmitri V Krysko,Marnik Vuylsteke,Peter Vandenabeele,Tom Vanden Berghe

NATURE PROTOCOLS（2016）

引用 44|浏览22

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摘要

This protocol describes a fluorescence-based assay to measure cell death rate and type (either caspase-dependent apoptosis or caspase-independent necrosis). The method allows for the detection of modality switches between apoptosis and necrosis over time.

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关键词

Cell death,Fluorescent dyes,High-throughput screening

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