Generation of knockout mice by Cpf1-mediated gene targeting

To the Editor: Engineered nucleases, including transcription activator-like effector nucleases (TALENs) and CRISPR-Cas9, are under active development as versatile genome-editing tools in various organisms including mice1, 2, 3. Recently, diverse Cpf1 orthologs were identified as representatives of a class 2 CRISPR-Cas system in vitro and showed target-specific endonuclease activities in human… Download references