Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases
NATURE PROTOCOLS(2016)
摘要
Genome editing using designer nucleases such as TALENs or the CRISPR-Cas9 system is hampered by a lack of methods to detect and quantify the products. Here the authors present GEF-dPCR, a droplet-based digital PCR method for assessing gene-editing frequencies.
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关键词
RNA sequencing,RNA splicing,Transcriptomics,Transcription
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