Interfacing 3D magnetic twisting cytometry with confocal fluorescence microscopy to image force responses in living cells

Yuejin Zhang,Fuxiang Wei,Yeh-Chuin Poh,Qiong Jia,Junjian Chen,Junwei Chen,Junyu Luo,Wenting Yao,Wenwen Zhou,Wei Huang,Fang Yang,Yao Zhang,Ning Wang

NATURE PROTOCOLS（2017）

引用 39|浏览27

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摘要

Mechanical forces can trigger a variety of biological responses in cells and tissues. This protocol describes how to combine 3D magnetic twisting cytometry with confocal fluorescence microscopy to study these force responses in greater detail.

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关键词

Biophysics,Confocal microscopy,Mechanotransduction,Super-resolution microscopy,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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