FRET monitoring of a nonribosomal peptide synthetase

Jonas Alfermann,Xun Sun,Florian Mayerthaler,Thomas E Morrell,Eva Dehling,Gerrit Volkmann,Tamiki Komatsuzaki,Haw Yang,Henning D Mootz

NATURE CHEMICAL BIOLOGY（2017）

引用 25|浏览23

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摘要

FRET sensors based on the adenylation and peptidyl carrier protein domains of a nonribosomal peptide synthetase illuminate the relationships between conformational dynamics and the catalytic cycle of this multidomain assembly line enzyme.

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关键词

Biophysical chemistry,Biosynthesis,Enzymes,Structural biology,Chemistry/Food Science,general,Biochemical Engineering,Biochemistry,Cell Biology,Bioorganic Chemistry

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