Croceitalea Marina Sp Nov., Isolated From Marine Particles Of Yellow Sea, And Emended Description Of The Genera Croceitalea

A Gram-staining-negative, rod-shaped, non-motile, golden yellow-coloured and strictly aerobic bacterial strain, designated H01-35(T), was isolated from a surface marine particles sample collected from the Yellow Sea in China. According to the phylogenetic analysis based on 16S rRNA gene sequences, the strain H01-35(T) belonged to the genus Croceitalea and showed the highest sequence similarity to Croceitalea litorea CBA3205(T) (96.4 %). Strain H01-35(T) grew optimally at pH 8.0-9.0, 28 degrees C and in the presence of 3% (w/v) NaCl. The DNA G+C content was 52.7 mol%. Strain H01-35(T) contained MK-6 as the predominant respiratory quinone and held iso-C-15:1 G, iso-C-15:0 and iso-C-17:0 3-OH as the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. Exoenzymes for starch, gelatin and Tween 20 degradation were detected in Strain H01-35(T) but the strain was negative for sulfur and indole production. On the basis of the polyphasic analyses, this isolate was considered to represent a novel species in the genus Croceitalea, for which the name Croceitalea marina sp. nov. is proposed. The type strain is H01-35(T) (MCCC 1K03229(T)=KCTC 52368(T)). The emendation of description of the genus Croceitalea is also given.