Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.

S H Andersen,L Vervelde,K Sutton,L R Norup,E Wattrang,H R Juul-Madsen,T S Dalgaard

Veterinary Immunology and Immunopathology（2017）

引用 18|浏览18

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摘要

•An avian ICS assay for detection of chIFN-γ was established.•Commercially available chIFN-γ antibodies were evaluated using tranfected CHO cells.•Functional T cell responses were addressed in NDV vaccination study.•Circulating T cells producing IFN-γ were quantified and phenotyped by flow cytometry.

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关键词

Chicken,Vaccines,Intracellular cytokine staining,Interferon-γ,Flow cytometry,T cells

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