Non-targeted metabolomics-guided sildenafil metabolism study in human liver microsomes.

•We revisited sildenafil metabolism in human liver microsomes using a metabolomics approach.•12 metabolites were identified in HLMs, and elucidated by MS2 fragmentation using HR-MS system.•7 Novel metabolites of sildenafil were identified in human liver microsomes.•The global metabolic picture of sildenafil were performed by HR-MS and multivariate data analysis.