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Simultaneous Site-Directed Mutagenesis of Duplicated Loci in Soybean Using a Single Guide RNA

Yuhei Kanazashi,Aya Hirose, Ippei Takahashi,Masafumi Mikami,Masaki Endo,Sakiko Hirose,Seiichi Toki,Akito Kaga,Ken Naito,Masao Ishimoto,Jun Abe,Tetsuya Yamada

Plant cell reports（2018）

Cited 65|Views7
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Abstract
Using a gRNA and Agrobacterium-mediated transformation, we performed simultaneous site-directed mutagenesis of two GmPPD loci in soybean. Mutations in GmPPD loci were confirmed in at least 33% of T2 seeds.
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Key words
CRISPR/Cas9,Generation,Glycine max,Heritable mutation,Null-segregant,PEAPOD
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