Practical Experience with Hole-Free Phase Plates for Cryo Electron Microscopy.
MICROSCOPY AND MICROANALYSIS(2016)
摘要
Phase plate (PP) imaging has proven to be valuable in transmission cryo electron microscopy of unstained, native-state biological specimens. Many PP types have been described, however until the recent implementation of the "hole-free" phase plate (HFPP), imaging has been challenging. We found the HFPP to be simple to construct and to set up in the transmission electron microscopy, but care in implementing automated data collection is needed. Performance may be variable, both initially and over time, thus it is important to monitor and evaluate image quality by observing the power spectrum. We found that while some HFPPs gave transfer to high resolution without CTF oscillation, most reached high resolution when operated with modest defocus.
更多查看译文
关键词
phase plate,contrast-transfer function,cryo electron tomography (cryo-ET),cryo electron microscopy (cryo-EM),SerialEM
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要