Antibody-free detection of infectious bacteria using quantum dots-based barcode assay.

Kristyna Cihalova,Dagmar Hegerova,Ana Maria Jimenez,Vedran Milosavljevic,Jiri Kudr,Sylvie Skalickova,David Hynek,Pavel Kopel,Marketa Vaculovicova,Vojtech Adam

Journal of Pharmaceutical and Biomedical Analysis（2017）

引用 35|浏览4

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摘要

•Detection of S. aureus, methicillin-resistant S. aureus and K. pneumoniae.•The method was able to detect as low concentrations of bacteria as 102 CFU/mL.•Detection was enabled using the bacteria-specific genes (fnbA, mecA and wcaG).•The proposed method is employing quantum dots and magnetic particles.

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关键词

Staphylococcus aureus,Methicillin-resistant Staphylococcus aureus,Klebsiella pneumoniae,Magnetic particles,Barcode,Quantum dots

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