The EB66® cell line as a valuable cell substrate for MVA-based vaccines production.

Arnaud Léon, Anne-Laure David, Brice Madeline, Laurence Guianvarc'h, Elodie Dureau,Patrick Champion-Arnaud,Matthias Hebben, Thierry Huss,Benoît Chatrenet,Klaus Schwamborn

Vaccine（2016）

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摘要

•EB66® cell line is a valuable cell substrate for production of MVA-based vaccine.•Pilot-scale production process was established that yields high titres.•Pilot-scale purification process has been developed.•Virus attenuation and transgene expression are maintained in EB66® cells.

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CEF,CEV,DMEM,DNA,ELISA,EMEM,FBS,GFP,GMP,HCD,HCP,HCV,HDF,IGBMC,MGB,MOI,MRC,MVA,PESU,qPCR,TCID50,TFF,VACV107

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