Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells.

Marie Holt,Anne Costanzo,Louis Gioia,Brian Abe,Andrew I Su,Luc Teyton

Methods in molecular biology (Clifton, N.J.)（2018）

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摘要

The paucity of pathogenic T cells in circulating blood limits the information delivered by bulk analysis. Toward diagnosis and monitoring of treatments of autoimmune diseases, we have devised single-cell analysis approaches capable of identifying and characterizing rare circulating CD4 T cells.

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关键词

Autoimmunity,CD4 T cells,Microfluidics,Peripheral blood,Single-cell analysis,TCR sequences

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