Single-cell microscopy of suspension cultures using a microfluidics-assisted cell screening platform

Burak Okumus,Charles J Baker,Juan Carlos Arias-Castro,Ghee Chuan Lai,Emanuele Leoncini,Somenath Bakshi,Scott Luro,Dirk Landgraf,Johan Paulsson

NATURE PROTOCOLS（2017）

引用 21|浏览19

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摘要

This protocol describes a microfluidics platform, termed 'MACS', for single-cell microscopy of suspension cells. MACS temporarily immobilizes the cells, allowing for high-throughput and high-resolution imaging, cell sorting, and single-molecule detection.

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关键词

Cellular imaging,Microfluidics,Single-molecule biophysics,Total internal reflection microscopy,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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