Molecular Characterisation of Carbapenem-Resistant Enterobacter Cloacae Complex in Colombia: Blakpc and the 'changing Landscape'.

Objectives: The aim of this study was to examine the population structure of representative carbapenem-resistant Enterobacter cloacae complex (CR-Ecl) isolates from eight different Colombian regions and to characterise their associated beta-lactamases. Methods: A total of 28 CR-Ecl isolates collected in Colombia between 2009-2013 through the Colombian Nosocomial Network were included in this study. Antimicrobial susceptibility testing was performed by the broth microdilution method. Molecular detection of carbapenemase and extended-spectrum beta-lactamase (ESBL) genes and the presence of transposon Tn4401 was evaluated by PCR and DNA sequencing. Genetic relatedness was assessed by multilocus sequencing typing (MLST) and repetitive sequence-based PCR (rep-PCR). Results: PCR and DNA sequencing revealed that 19/28 (68%) of the CR-Ecl isolates carried bla(KPC-2). Analysis of the genetic environment found bla(KPC-2) within transposon Tn4401b in 8/19 isolates (42%). Population genetic analysis using rep-PCR revealed four clonal groups. MLST showed a variety of sequence types (STs), among which ST510 was the most common (10/28 isolates; 36%). Conclusions: bla(KPC-2) was discovered as the most common mechanism of carbapenem resistance in CR-Ecl and was disseminated among different STs. Although none of the previously reported major clonal complexes were identified, it appears that local strain lineages are associated with the spread of bla(KPC-2) within CR-Ecl in various regions of Colombia. (C) 2017 Published by Elsevier Ltd on behalf of International Society for Chemotherapy of Infection and Cancer.