Topical Application Of Glycyrrhetinic Acid In The Gingival Sulcus Inhibits Attachment Loss In Lipopolysaccharide-Induced Experimental Periodontitis In Rats

Background and Objective: Attachment loss of the junctional epithelium and alveolar bone destruction are signs of periodontitis, which is mainly caused by an inflammatory response to dental plaque. Glycyrrhetinic acid (GA), a component of the licorice herb, has been shown to have important anti-inflammatory activities; however, there are no previous reports on the ability of its inhibitory effects to prevent periodontal diseases. Hence, in this study, using our experimental periodontitis model, we attempted to evaluate whether GA had an effect on the prevention of attachment loss and alveolar bone loss.Material and Methods: Rats were intraperitoneally immunized with Escherichia coli lipopolysaccharide (LPS). The LPS group (n=5) received 3 topical applications of 50 mu g/mu L of LPS followed by one application of the vehicle (propylene glycol:ethyl alcohol:phosphate-buffered saline [PBS]=8:1:1) into the gingival sulcus. This protocol was repeated twice per day for 10days. The low (n=5) and high (n=5) groups received topical application of LPS and 0.03% or 0.3% GA, respectively. The control group received topical application of PBS and vehicle. The rats were killed on the 10th day. Attachment loss, alveolar bone level and inflammatory cell infiltration were investigated histometrically. The formation of immune complexes and infiltration of LPS were evaluated immunohistologically.Results: Attachment loss, formation of immune complexes and infiltration of inflammatory cells were increased in the LPS group compared with the control group, and were completely inhibited in the low and high groups compared with the LPS group. The LPS group showed greater alveolar bone destruction compared with the control group and GA-treated groups. In addition, invasion of LPS was detected in the LPS group, was absent in the control group and was weaker in the GA-treated groups than in the LPS group.Conclusion: In the present study, we showed that GA inhibits periodontal destruction in the rat experimental periodontitis model.