A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses.

Ruixing Wang,Sophie Brustlein,Sébastien Mailfert,Roxane Fabre,Mathieu Fallet,Siddharth Sivankutty,Hervé Rigneault,Didier Marguet

Methods (San Diego, Calif.)（2018）

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摘要

•A theoretical analysis of the incomplete depletion of fluorescence in STED-FCS process.•A numerical analysis of the STED effective fluorescent volume.•The performances of different typical fitting models are compared.•A STED-background corrected fitting model is proposed to retrieve unbiased diffusion times and average molecule numbers.•A validation of the new fitting model on experimental STED-FCS measurements is provided.

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关键词

Fluorescence correlation spectroscopy,Stimulated emission depletion,Fluorescence auto-correlation fitting model,non-Gaussian-shaped PSF

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