Flavobacterium Ovatum Sp Nov., A Marine Bacterium Isolated From An Antarctic Intertidal Sandy Beach

A rod-shaped, Gram-staining-negative, strictly aerobic, non-motile bacterium with no flexirubin-type pigment, designated as W201E(T), was isolated from an intertidal sandy beach in Antarctica. The organism formed faintly yellow, round colonies on marine agar 2216E. The strain required sea salts for growth and grew optimally in the presence of 2 % (w/v) NaCl at pH 7.0, 20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain W201E(T) belonged to the genus Flavobacterium and showed the highest sequence similarity to Flavobacterium algicola NBRC 102673(T) (96.5 %). The major respiratory quinone was menaquinone 6, and the predominant fatty acids were iso-C-15:1 G, iso-C-15:0, iso-C(15:0 )3-OH and summed feature 3 (which comprises C-16(:1) omega 7c and/or C-16:1 omega 6c). The polar lipids of strain W201E(T) comprised one phosphatidylethanolamine, two unidentified aminolipids and three unidentified polar lipids. The DNA G+C content of strain W201E(T) was 34.1 mol%. On the basis of the polyphasic analyses, this isolate was considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium ovatum sp. nov. is proposed. The type strain is W201E(T) (=KCTC 52693(T) =MCCC 1K03251(T)=CGMCC 1.16053(T)).