Studying the Role of AMPK in Autophagy.
AMPK: METHODS AND PROTOCOLS（2018）
Abstract
AMPK is an energy-sensing kinase and is required for the induction and progression of the autophagy process. In this chapter, we describe experimental approaches to study the steady state and flux of autophagy in response to AMPK activation. For this purpose, we provide detailed protocols for the measurement of general as well as AMPK-specific autophagy markers by immunoblot and immunofluorescence analysis.
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Key words
A-769662,AICAR,AMP-activated protein kinase,Autophagy,Bafilomycin A1,Flux analysis,Glucose deprivation,Immunoblot,Immunofluorescence,LC3
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