A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species

Mi-Ra Kim,Kisung Kwon,Yoo-Kyung Jung,Tae Sun Kang

Food Chemistry（2018）

引用 26|浏览3

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摘要

•Sensitive Beringraja pulchra-specific and skate-universal primer sets were designed.•Accurate and reliable qPCR method was developed for identification of skate species.•Ultra-fast qPCR assay within 30 min was also developed for on-site identification.

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关键词

Genetic identification,Seafood authentication,Ultra-fast qPCR,Cytochrome oxidase subunit I,Amplification efficiency

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