Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6.

Yoshinori Shimamoto,Junko Nio-Kobayashi,Hiroshi Watarai,Masashi Nagano,Natsuko Saito,Eiki Takahashi,Hidetoshi Higuchi,Atsushi Kobayashi,Takashi Kimura,Hiroshi Kitamura

Veterinary Immunology and Immunopathology（2018）

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摘要

•Western blot analysis using ABM-1A9 and ABM-2D6 detected bovine CD163 with high sensitivity.•ABM-1A9 and ABM-2D6 stained phagocytic macrophages in formalin-fixed sections.•ELISA using ABM-2D6 is applicable in measuring circulating soluble bovine CD163.

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关键词

CD163,M2 macrophage,Monoclonal antibody,Inflammation,Immunohistochemistry,ELISA

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