Flavobacterium Aurantiibacter Sp Nov., An Orange-Pigmented Bacterium Isolated From Cyanobacterial Aggregates In A Eutrophic Lake

A bacterial strain, designated TH167(T), was isolated from cyanobacterial aggregates in eutrophic Lake Taihu in China. Cells were observed to be slightly curved rod-shaped, motile by gliding, aerobic, Gram-stain-negative, proteorhodopsin-containing. Optimal growth was obtained at pH 7.0 (range: 6.0-9.0), 28 degrees C (range: 4-32 degrees C) and 0 % (w/v) NaCl (range: 0-2.0 %) in Reasoner's 2A broth. No growth was observed at 37 degrees C. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as iso-C-15( : 0), iso-C-15( : 1) G and anteiso-C-15( : 0). The major polar lipids of the isolate comprised phosphatidylethanolamine, one unidentified phospholipid and two unidentified aminolipids. The major respiratory quinone was menaquinone-6. The genomic G+C content of strain TH167(T) was 40.4 mol% based on total genome calculations. Based on similarities of 16S rRNA gene sequences, strain TH167(T) was affiliated with the genus Flavobacterium, exhibiting the highest sequence similarities to Flavobacterium eburneum SA31(T) (94.16 %), Flavobacterium yanchengense hg(T) (94.09 %) and Flavobacterium lacus NP180(T) (93.95 %). The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH167(T) represented a novel species within the genus Flavobacterium, for which the name Flavobacterium aurantiibacter sp. nov. is proposed. The type strain is TH167(T) (=CGMCC 1.15805(T)=LMG 29719(T)).