Isolation and functional assessment of mouse skeletal stem cell lineage

Gunsagar S Gulati,Matthew P Murphy,Owen Marecic,Michael Lopez,Rachel E Brewer,Lauren S Koepke,Anoop Manjunath,Ryan C Ransom,Ankit Salhotra,Irving L Weissman,Michael T Longaker,Charles K F Chan

NATURE PROTOCOLS（2018）

引用 52|浏览30

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摘要

flow cytometry–based approach using eight surface markers is used to distinguish cells of the skeletal stem cell lineage. Renal subcapsular transplantation and in vitro colony-formation assays are also described for cell characterization.

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关键词

Flow cytometry,Multipotent stem cells,Musculoskeletal models,Stem-cell differentiation,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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