Identification And Characterization Of A Novel Bacterial -Glucosidase That Is Highly Specific For The-1,2-Glucosidic Linkage Of Sesaminol Triglucoside

A gene (PSTG2) coding for a novel -glucosidase belonging to glycoside hydrolase family 3 was identified in the vicinity of the previously identified -glucosidase gene [sesaminol triglucoside (STG)-hydrolyzing -glucosidase, PSTG1] in the genome of Paenibacillus sp. strain KB0549. Compared with PSTG1, recombinant PSTG2 more specifically acted on the -1,2-glucosidic linkage of the STG molecule to transiently accumulate a larger amount of 6-O-(-D-glucopyranosyl)--D-glucopyranosylsesaminol.