Influence of mediators on laccase catalyzed radical formation in lignin.

Line Munk,Mogens Larsen Andersen,Anne S Meyer

Enzyme and Microbial Technology（2018）

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摘要

•Time course EPR measurements of radical formation kinetics by laccase-mediator systems on lignin.•N-OH type mediators HPI and HBT do not affect laccase catalyzed radical formation rates on lignin.•High doses of N-OH mediators in laccase-mediator systems decrease radical formation in lignin relative to laccase treatment.•Electron shuttling by mediators is apparently not a general mechanism enhancing laccase catalyzed oxidation of lignin.

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关键词

Electron paramagnetic resonance (EPR),Trametes versicolor,Myceliophthora thermophila,HBT,HPI,TEMPO,ABTS,Laccase-mediator systems

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