Multi-laboratory comparison of three commercially available Zika IgM enzyme-linked immunosorbent assays.

Alison Jane Basile,Christin Goodman,Kalanthe Horiuchi,Angela Sloan,Barbara W Johnson,Olga Kosoy,Janeen Laven,Amanda J Panella, Isabel Sheets,Freddy Medina,Emelissa J Mendoza, Monica Epperson,Panagiotis Maniatis,Vera Semenova,Evelene Steward-Clark,Emily Wong,Brad J Biggerstaff,Robert Lanciotti,Michael Drebot,David Safronetz,Jarad Schiffer

Journal of Virological Methods（2018）

引用 19|浏览34

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摘要

•ZIKV IgM kits from InBios, NovaTec and Euroimmun were compared in 3 separate labs.•InBios kit was most sensitive; Novatec and Euroimmun kits were most specific.•Concordance across the labs was high for both the NovaTec and Euroimmun IgM + IgG kits.•The NS1-based NovaTec and Euroimmun kits may be useful in confirmation of infection for a portion of patients and to reduce the number of PRNT’s required.

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关键词

Zika,Dengue,Virus,Kits,ELISA,Enzyme-linked immunosorbent assay,Commercial,IgM,IgG,Immunoglobulin M,Immunoglobulin G,Comparison,Nonstructural protein 1,NS1,Envelope E

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