Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice

Weijian Zong,Runlong Wu,Mingli Li,Yanhui Hu,Yijun Li,Jinghang Li,Hao Rong,Haitao Wu,Yangyang Xu,Yang Lu,Hongbo Jia,Ming Fan,Zhuan Zhou,Yunfeng Zhang,Aimin Wang,Liangyi Chen,Heping Cheng

NATURE METHODS（2017）

引用 334|浏览65

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摘要

FHIRM-TPM is a miniature two-photon microscope capable of imaging fluorescently labeled neurons in the brains of freely behaving mice. It allows for imaging of spines or recording of neural activity with a frame rate up to 40 Hz.

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关键词

Ca2+ imaging,Fluorescence imaging,Mouse,Multiphoton microscopy,Neuroscience,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics

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