Transgenerational effects of heavy fuel oil on the sea urchin Strongylocentrotus intermedius considering oxidative stress biomarkers

Stranding of oil onto a coastline after an oil spill threatens the health of marine benthic organisms. Here, the transgenerational effects of exposure to stranded heavy fuel oil (HFO) on the sea urchin Strongylocentrotus intermedius were assessed. The column containing gravel coated with HFO was prepared in the laboratory to simulate HFO-contaminated gravel shorelines. Adult sea urchins were exposed for 21 days to either a HFO-oiled gravel column at the oil loading of 3000 μg oil/g gravel or a non-HFO-oiled gravel column (as the control treatment) and then offspring were either exposed to HFO or ambient seawater conditions. The sublethal exposure to HFO for 21 days induced polycyclic aromatic hydrocarbons (PAHs) accumulation in gonads, accompanied by increased levels of oxidative lipid, protein and DNA damage and a reduction in total antioxidant capacity. Analysis of gametes indicated that both maternal and paternal exposure could result in the transfer of PAHs and DNA damage to their offspring. Parental (maternal, paternal or both) exposure to HFO caused increases in malformation rates of offspring compared to those from control parents under ambient seawater condition. Continued HFO exposure in the offspring resulted in further increased malformation rates compared with those reared in ambient seawater, as well as oxidative lipid, protein and DNA damage. Furthermore, mother exposure history reduced the total antioxidant capacity of larvae to response to continued HFO exposure. Overall, the results suggest an increased sensitivity to toxic effects of HFO in larvae from exposed both parents compared with those from control parents in S. intermedius, which may consequently affect the recruitment and population maintenance.