All-Night Functional Magnetic Resonance Imaging Sleep Studies.

•This is a validation of procedures for obtaining all-night fMRI data in sleeping subjects without sleep deprivation.•Subjects slept in the scanner on two consecutive nights, allowing the first night to serve as an adaptation night.•Performing all-night fMRI sleep studies is feasible.•Because the two nights of the study were performed consecutively, some sleep deprivation from Night 1 may have occurred.•It is envisioned that other laboratories can adopt the core features of this protocol to obtain similar results.