Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells.

•Multi-primed quantitative RT-PCR is suitable for accurate quantification of RNA molecules enabling standardisation.•Viral RNA profiling revealed the expected RNA expression patterns according to the latency program and presence of reactivating EBV.•Quantification of 16 viral targets enables monitoring of EBV behaviour in malignancies during therapy as biomarkers for therapy response.