Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry.
Methods in molecular biology (Clifton, N.J.)(2019)
摘要
Neo-antigens expressed on tumors are targets for development of cancer immunotherapy strategies. Use of prediction algorithms to identify neo-antigens yields a significant number of peptides that must be validated in laborious and time-consuming methods; many prove to be false-positive identifications. The use of HLA peptidomics allows the isolation of the HLA-peptide complexes directly from cells and can be done on fresh tumor, patient-derived xerographs, or cell lines when the tissue sample is limited. This method can be used to identify both HLA class I and HLA class II or any different MHC from different species. Here we describe the steps to create the immune-affinity columns used from the process, the immunoprecipitation procedure, and also the isolation of the peptides that will be analyzed by mass spectrometry.
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关键词
Exome sequencing,HLA peptidomics,MHC peptides,Mass spectrometry,Neo-antigens
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