A structure based plasma protein pre-fractionation using conjoint immobilized metal/chelate affinity (IMA) system.

Karan Karkra,Kishore K R Tetala,M A Vijayalakshmi

Journal of Chromatography B（2017）

引用 10|浏览4

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摘要

•Conjoint IMA for plasma protein pre-fractionation prior to LC–MS/MS in proteomics.•Fractionate proteins in their native state based on their surface “His” topography.•Single step protein pre-fractionation system using single buffer system.•3 fold proteins identified with this strategy compared to without-fractionation.

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关键词

CIM disk,Conjoint IMA,Pre-fractionation,Proteins,Plasma

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