Quantitation of fumonisin B1 and B2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection

Lori L Smith,Kyle A Francis,Joseph T Johnson,Cynthia L Gaskill

Food Chemistry（2017）

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摘要

•FMOC-derivatized fumonisin calibrants experience no fluorescent decay over 7days.•Immunoaffinity SPE clean-up eliminated matrix effects in corn-based feed extracts.•Quantitative results indicate FMOC-Cl is a suitable alternative reagent to OPA.

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关键词

Fumonisin B1 (PubChem CID: 3431),Fumonisin B2 (PubChem CID: 3432),Fluorenylmethyl chloroformate (PubChem CID: 34367)

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