[Expression profile of Notch signaling pathway-related microRNAs in immune cells of collagen-induced arthritis mice].

Objective To study the expression of Notch signaling pathway-related microRNAs in T cells of collagen-induced arthritis (CIA) mice. Methods First, the mice model was established with bovine collagen type II and immune adjuvant DBA/1 J mice. Next, peripheral blood mononuclear cells (PBMCs) were isolated and CD4+ T and CD8+ T cells were sorted from the peripheral blood, spleen, lymph node in CIA mice by using flow cytometry. Finally, real-time quantitative PCR was used to detect the expression levels of Notch signaling pathway-related microRNAs in PBMCs and T cells. Results The levels of miR-200b-3p, miR-30c-5p, miR-30b-5p and miR-23b-3p in the PBMCs of CIA mice were significantly decreased; miR-200b-3p, miR-30c-5p and miR-30b-5p in peripheral blood, spleen, lymph node CD4+ T cells and CD8+ T cells of CIA mice was not significantly different from that of normal controls; but the expression level of miR-23b-3p in peripheral blood and spleen CD4+ T cells of CIA mice was significantly lower than that of normal control mice. Conclusion miR-23b-3p may be involved in the immunoregulation of Notch signaling to T cells of CIA mice.